In fact, one of many typical complications of Y2H is definitely the technology of Phony positives due to non-specific interactions (as explained in detail more underneath). Assortment for 2 Energetic reporter genes demands a additional good transcriptional activation and thus raises the stringency of your assay, but concomitantly penalizes detection https://www.directivepublications.org/the-journal-of-molecular-biology/