The cDNA sequence of human NAMPT was amplified by PCR from a pGex-6p-three-hNAMPT plasmid (kindly gifted from Dr Shui-qing YE, University of Missouri). The PCR products were being digested and cloned into a pET21a+ vector utilizing NdeI and XhoI restriction enzymes. A degree mutation was launched by the short modify https://elizabetho776zky0.wikipublicity.com/user